5-Amino-1MQ is a small-molecule nicotinamide N-methyltransferase (NNMT) inhibitor widely used for in vitro cellular metabolism studies. This research guide provides an overview of its pharmacological properties, mechanisms of action, and applications strictly in laboratory research settings. It is essential to emphasize that 5-Amino-1MQ is intended for research use only (RUO) and is not approved for use in humans or animals. All studies should adhere to relevant ethical and safety guidelines when handling this compound in cell-based assays.
What is 5-Amino-1MQ and its Role as an NNMT Inhibitor?
5-Amino-1MQ, or 5-amino-1-methylquinolinium, is a potent inhibitor of NNMT, an enzyme involved in nicotinamide metabolism. Chemically, it has the formula C9H11N3 and a molecular weight of approximately 161.2 Da. The compound competitively binds to NNMT’s active site, thereby reducing enzyme activity, which enables researchers to study downstream metabolic changes related to NAD+ modulation and mitochondrial function in vitro. The inhibitory concentration (IC50) is approximately 100 nM, with a purity of 99%, verified by high-performance liquid chromatography (HPLC). For more information and to obtain high-purity 5-Amino-1MQ for research, visit the official product page.
Key in vitro research demonstrates that NNMT inhibition affects metabolic regulation pathways in cultured cells. The specific mechanisms include modulation of SAM and NAD+ levels and regulation of histone methylation, relevant to metabolic gene expression studies.
NNMT Inhibition: Mechanisms in Cellular Metabolism NNMT expression is upregulated in adipose and hepatic tissues under metabolic stress conditions. Knockdown of NNMT in cell models leads to increased cellular energy expenditure and altered methylation states, impacting metabolic gene regulation. Nicotinamide N-methyltransferase knockdown protects against metabolic perturbations in vitro, D Kraus, 2014
Research Applications of NNMT Inhibition with 5-Amino-1MQ
5-Amino-1MQ is utilized predominantly in cell-based assays to elucidate NNMT’s role in metabolic pathways, including:
- Cellular Metabolism Studies: Investigation of NAD+ biosynthesis and mitochondrial function in vitro.
- Metabolic Disorder Models: Elucidating NNMT’s involvement in metabolic enzyme regulation within cultured cells.
- Oncology Research: Exploring NNMT’s role in cancer cell metabolism and apoptotic mechanisms in cell lines.
The compound is a valuable research tool for academic and institutional laboratories focused on metabolic biochemistry. Researchers are advised to refer to the catalog for available research peptides and related compounds and the Trustpilot reviews for supplier reliability.
Restoring NAD+ Homeostasis in Autophagic Deficiency Models NAD+ deficiency due to autophagy impairment is linked to induction of NNMT, which methylates nicotinamide to MeNAM. Inhibition of NNMT activity with 5-Amino-1MQ in cardiomyocyte cultures restores NAD+ levels, ameliorating mitochondrial dysfunction. Control of NAD+ homeostasis modulates mitochondrial and cardiac cell function, Q Zhang, 2024
Research Use Only (RUO) Compliance and Safety Guidelines
Important: 5-Amino-1MQ is intended exclusively for in vitro research use. It is confined strictly to non-clinical, in vitro laboratory settings and authorized research protocols. Researchers must ensure all necessary institutional approvals and safety protocols are in place before use.
Recommended in vitro experimental concentrations typically range from 1 to 50 μM. In vivo or clinical dosing references are strictly excluded from this guide. Appropriate laboratory personal protective equipment (PPE) and handling procedures must be observed.
Quality Standards and Supply Information for 5-Amino-1MQ
5-Amino-1MQ is supplied in tablet form, with 50 mg per tablet in bottles of 25 tablets. The product undergoes batch conformity testing, ensuring less than 0.5% variance in purity across eight vials, verified by HPLC and ESI-MS. Additionally, a comprehensive 4-point safety screening panel is performed. Orders placed before 2 PM EST benefit from domestic same-day dispatch. Payments are processed directly via credit card for researcher convenience.
For procurement, quality assurance, and comprehensive product details, researchers should consult the 5-Amino-1MQ product page and company catalog.
This guide emphasizes the strictly in vitro research application of 5-Amino-1MQ as an NNMT inhibitor, highlighting high-quality control standards and strict RUO compliance to ensure safe and effective metabolic studies in cellular models.
This article is for informational and research purposes only. All products discussed are sold strictly for laboratory and research use, not for human or veterinary use, consumption, or diagnostic application.
